TABLE 1.

Dissociation constant, stoichiometry coefficient, enthalpy change, Gibbs free energy change, and entropy change of the cdM2-1/RNA interaction, determined using fluorescence-quenching experiments at 15, 25, and 35°C^a

Temp (°C)	Kd (10−6 M)	nSC	ΔH (kJ · mol−1)	ΔG (kJ · mol−1)	ΔS (J · mol−1 · K−1)
15	0.43 ± 0.18	2.0 ± 0.4	−45 ± 10	−35.1 ± 0.7	−34 ± 35
25	1.24 ± 0.18	3.3 ± 1.1		−33.7 ± 0.4	−37 ± 34
35	1.70 ± 0.28	1.4 ± 0.2		−34.0 ± 0.4	−36 ± 32

^aAll experiments were performed in 50 mM phosphate buffer (pH 6.5) containing 150 mM NaCl and 1.0 mM DTT. K_d, dissociation constant; n_SC, stoichiometry coefficient; ΔH, enthalpy change; ΔG, Gibbs free energy change; ΔS, entropy change.