FIG 2.
ZIKV replication is restored in IRE1α-rescuing cells. (A to C) Viral replication levels in IRE1αRes A549 cells. Control cells, IRE1αKo cells, and IRE1αRes cells were infected with ZIKV at an MOI of 3 and harvested at 24 h p.i. for qRT-PCR (A) or Western blotting (B). The supernatants were collected at 24 h p.i. for plaque assay (C). Human β-ACTIN mRNA level was measured as an internal control for qRT-PCR. Data are shown as means ± SD from at least three independent experiments. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (unpaired, two-tailed Student t test).