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. 2020 Nov 9;94(23):e01229-20. doi: 10.1128/JVI.01229-20

TABLE 3.

Sequences of primers used in CRISPR/Cas9 gene editing for IRE1α

Primer Sequence (5′–3′) Targeting region
sgIRE1α-1-5F CACCGCCAAAACTACGCCTCCCCTG Exon 4
sgIRE1α-1-3R AAACCAGGGGAGGCGTAGTTTTGGC
sgIRE1α-2-5F CACCGAGAGGACAGGCTCAATCAAA Exon 2
sgIRE1α-2-3R AAACTTTGATTGAGCCTGTCCTCTC