FIG 4.

Chelating activity of SMALPs causes inhibition of DNA-modifying reactions. (a) DNase inhibition by SMALPs, as shown by an agarose gel with λ DNA with SMALPs and DNase, λ DNA with DNase, and λ DNA alone. (b) Inhibition of Taq polymerase by SMALPs after PCR targeting λ DNA. (c) Precipitation of SMA by addition of divalent cations in calcium chloride (Ca) and magnesium sulfate (Mg), followed by centrifugation. SMA concentration was determined from the adsorption at 259 nm and by comparison to a standard curve (see Fig. S2 in the supplemental material). Points indicate replicates; solid lines indicate averages.