Figure 4.

Baricitinib treatment abolishes inflammatory cytokine and neutrophil chemoattractant expression in bronchoalveolar macrophages

Single-cell suspensions from BALs of SARS-CoV-2-infected RMs were subject to 10× Genomics capture and sequencing.

(A) UMAP showing major cell types in BAL samples (n = 10 samples; untreated, baseline n = 3; untreated, 4 days after infection n = 3; treated, baseline n = 2; treated, 4 days after infection n=2).

(B) UMAP showing clusters in BAL samples by treatment days (n = 10).

(C) UMAP projection of pro-inflammatory cytokines in macrophages.

(D) UMAP projection of neutrophil chemoattractant and pro-inflammatory chemokines.

(E and F) Expression of chemokines and interferon-stimulated genes (ISGs) in treated and untreated samples at baseline and 4 days after infection. The colored expression scale of expression in UMAPs is depicted on a per gene basis: the scale represents the per cell reads for each gene divided by the total reads for of that cell, scaled to the factor shown and natural log-transformed.

See also Figures S4 and S5.