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. Author manuscript; available in PMC: 2021 Jan 21.
Published in final edited form as: Sci Signal. 2020 Jul 21;13(641):eaaz0240. doi: 10.1126/scisignal.aaz0240

Fig. 5. Two kinase domain arrangements observed by cryo-EM.

Fig. 5.

(A) In reconstruction Class A, a single kinase domain was resolved between two hub domain subunits. A kinase domain from the CaMKIIα-0 holoenzyme crystal structure (PDB: 3SOA) was fitted into the additional density. (B) In a second reconstruction, Class B, a kinase domain was visible interacting with a hub subunit with the kinase C-lobe making contact with a loop at the top of the hub domain. The CaMKIIα kinase domain (PDB: 3SOA) was docked into the additional density, and the CaMKII hub domain (PDB: 5IG3) was docked into the hub density. (C) Unmasked maps of Class A are shown below in gray, where adjusted contour levels reveal EM density for additional kinase domains. (D) Sub-classification of Class B particles results in the 3D model shown in pink where EM density for two docked kinases is evident.