Table 3.
Effects of Topical Application of the Chloroform, Ethyl Acetate, and Aqueous Fractions of the Seeds of B. carinata on Wound Contraction of Excision Wound Model in Mice
| Days | Wound Area (mm2)±SEM | |||||||
|---|---|---|---|---|---|---|---|---|
| SO | 5% CF | 10% CF | 5% EAF | 10% EAF | 5% AQF | 10% AQF | NF | |
| 0 | 298.69±6.85 | 296.11±6.14 | 298.69±6.85 | 301.21±6.15 | 301.21±6.15 | 298.76±7.83 | 301.21± 6.15 | 298.69±6.85 |
| 2 | 283.65±7.7 | 268.86±6.49 | 266.41±5.83 | 264.02±6.12 | 259.18±4.84 | 263.96±4.83 | 261.57±4.95 | 261.57±4.95 |
| 4 | 261.76±8.06 | 245.25±6.86 | 224.94±7.25 | 203.35±6.81ad bc | 197.69±12.33ae bc | 201.22±6.49 ae bc | 195.76±13.12 ae bd | 203.35±6.81ad bc |
| 6 | 201.29±7.25 | 192.85±5.13 | 137.77±13.ea d | 133.19±7.45ad bd | 110.29±5.75ae be | 133.97±11.8adbd | 127.96±15.39ad bd | 150.85±8.43ac |
| 8 | 131.49±16 | 81.51±4.87ac | 74.05±6.62ad | 62.41±7.72ae | 48.54±3.82ae | 59.53±10.96ae | 53.81±16.3a3 | 79.02±5.74ac |
| 10 | 95.64±17.8 | 44.22±8.45ad | 36.63±7.22ad | 32.84±9.83ae | 21.393±2.4ae | 30.09±6.69ae | 19.53±5.73a3 | 41.47±5.97ad |
| 12 | 64.76±14.84 | 26.43±5.35ad | 13.35±3.18ae | 8.5±2.31ae | 8.54±2.7ae | 6.93±1.88ae | 3.04±0.95a3 | 23.03±3.7 ac |
| 14 | 33.62±10.65 | 10.34±2.38ad | 3.4±1.29ae | 1.7±1.19ae | 1.7±1.19ae | 1.05±0.66ae | 0 | 5.5±1.79ac |
| 16 | 26.82±8.53 | 2.75±1.07ae | 0.52±0.52ae | 0.52±0.52ae | 0 | 0 | 0 | 0.52±0.52 ac |
| 18 | 16.49±5.81 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Notes: Values are expressed as mean±SEM and analyzed by one-way ANOVA followed by Tuckey post-hoc test (n=6 animals in each group). 0 values refer to a day when complete healing was achieved; aAgainst control, bAgainst 5% (w/w) chloroform; cP<0.05, dP<0.01, eP<0.001.
Abbreviations: SO, simple ointment; CF, Chloroform Fraction, EAF, Ethyl Acetate Fraction, AQF, Aqueous Fraction, NF, Nitrofurazone.