Fig. 3.

Modulation of mucin production by fecal microbiota transplantation (FMT) in the proximal colon and distal colon of Na⁺/H⁺ exchanger isoform 8 knockout (NHE8KO) mice. A– D: periodic-acid Schiff (PAS) staining from the proximal colon and distal colon of NHE8KO mice receiving NHE8KO fecal material (KO/KO) comparing with NHE8KO mice receiving NHE8 wild-type (NHE8WT) fecal material (KO/WT). At least 10 crypts from each section were used for measurements, and the representative pictures were selected. E: number of goblet cells per crypt. F: goblet cell theca (µm²). G: Muc2 mRNA expression. PAS staining (×10) magnification. Periodic-acid Schiff (PAS)-positive cells were quantified using ImageJ program. Data are represented as means ± SE from 8 mice.