Fig. 1.
Relative expression of members of the Slc26 gene family and selected Cl− channel genes in mouse retinal pigment epithelium (RPE). Expression of genes is presented relative to the average of Actb and B2m expression levels. Quantitative RT-PCR was performed separately using 4 biological replicates of cDNA reversed transcribed from mRNA pooled from RNA isolated from the RPE of wild-type mice of the following ages and sexes: six 2-mo-old males, eight 2-mo-old females, eleven 3-mo-old males, and eleven 3-mo-old females. Symbols represent relative expression values for each of these pooled samples, and the vertical bars and error bars represent means ± SD (n = 4 experiments). The relative expression levels of Slc26a3, Slc26a5, and Slc26a9 were <2 × 10−5 and are not shown.