Fig. 4.

Effect of 140 mM thiocyanate (SCN⁻) on whole cell currents in an isolated Slc26a7 knockout mouse retinal pigment epithelial cell. The cell was dialyzed with an N-methyl-d-glucamine (NMDG)-Cl-based K⁺-free internal solution and superfused with a solution containing 140 mM NaCl (A) or 140 mM sodium thiocyanate (NaSCN; B). The effect of SCN⁻ was reversible (C). The family of currents in C was obtained after 10 min of superfusion with 140 mM NaCl solution to ensure the complete removal of SCN⁻. Currents were elicited using a voltage step protocol consisting of 1-s steps from −120 to +60 mV in intervals of 10 mV from a holding potential of 0 mV. Horizontal lines to the left of the current traces indicate the zero-current level.