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. 2020 Jul 29;319(4):C641–C656. doi: 10.1152/ajpcell.00027.2020

Fig. 7.

Fig. 7.

Exposure to 500 μM external thiocyanate (SCN−) produces little change in the electrophysiological properties of Slc26a7 knockout (KO) mouse retinal pigment epithelial (RPE) cells. A: families of whole cell currents evoked by voltage steps from a holding potential of −60 mV in an Slc26a7 KO RPE cell in the absence and presence of 500 μM external SCN−. Exposure to 500 μM SCN− produced small increases in inward and outward currents without changing their time course in contrast to the large transient currents that are observed in wild-type (WT) mouse RPE cells exposed to submillimolar SCN− (9). B: summary of experiments similar to those in A showing current-voltage (I-V) plots of instantaneous current obtained in the absence (closed circles) and presence (open circles) of 500 μM external SCN−. Symbols represent means, and bidirectional error bars represent SE (n = 11 cells from 5 mice of both sexes, 6–21 wk old). Five hundred micromolar SCN− produced small but significant increases in current at voltages in the range −114.5 to −84.5 mV and at +55.5 mV (P < 0.02; Šidák’s multiple comparisons test) but not at the other voltages tested (P > 0.05). C: comparison of normalized outward conductance of isolated Slc26a7 KO mouse (left) and WT mouse (right) RPE cells in the absence (open symbols) and presence (gray symbols) of 500 μM external SCN−. Horizontal lines and error bars represent means ± SD. The change in outward conductance produced by 500 μM SCN− was significantly smaller in Slc26a7 KO mouse RPE cells [0.010 ± 0.004 (SE) nS/pF, n = 11 cells from 5 mice of both sexes, 6–21 wk old] than in WT mouse RPE cells [0.173 ± 0.031 (SE) nS/pF, n = 7 cells from 2 female mice, 8 wk old; P < 0.005, unpaired t test]. Data for WT mouse RPE cells are from our previously published study on Slc26a7+/+ mice (9). *P < 0.05, **P < 0.005.