Table 1.

Target gene primer sequences and expected sizes of RT-PCR products

Target Gene	Primer Sequence	Expected Size, bp	GenBank Accession No.
Ano1	Forward 5′-tcgatcgtgggtgtcattgt-3′ Reverse 5′-acggtggcagggttatcaaa-3′	197	NM_178642
Ano2	Forward 5′-gtcccctgtgtgacaagtcc-3′ Reverse 5′-ttcgatcccagtcaggtccc-3′	183	NM_153589.2
Best1	Forward 5′-ggtgtggtttgccaacttgt-3′ Reverse 5′-cagaaactgcctcccgatca-3′	220	NM_011913
Best2	Forward 5′- gcacagggctacaaagacca-3′ Reverse 5′- gctagcatggacacctgg aa-3′	182	NM_001130194
Cftr	Forward 5′-gcatattgttgggaatcagc-3′ Reverse 5′-acgattccgttgatgactgt-3′	176	NM_021050
Clcn2	Forward 5′-taccacaggcaacatggagtc-3′ Reverse 5′-tggctcatctagctgctgttc-3′	217	NM_009900
Slc26a1	Forward 5′-ccaaccagctctctgtctgt-3′ Reverse 5′-gtcggaccagtaccagtgtc-3′	189	NM_174870
Slc26a2	Forward 5′-ctacataaagcctgccctga-3′ Reverse 5′-tgaaagaagcccattgctac-3′	182	NM_007885
Slc26a3	Forward 5′-gtcctcaagctccacagaaa-3′ Reverse 5′-ggcagctgtagcatgaattt-3′	216	NM_021353
Slc26a4	Forward 5′-aggagtgaggtcattgcgaa-3′ Reverse 5′-ctttacagtcccgaatgcga-3′	248	NM_011867
Slc26a5	Forward 5′-gaaaggcccatcttcagtcatc-3′ Reverse 5′-gccacttagtgataggcaggaac-3′	157	NM_030727
Slc26a6	Forward 5′-ttgctggagctgtatcttcc-3′ Reverse 5′-tgtttgccttccaaagagag-3′	162	NM_134420
Slc26a7	Forward 5′-gcatgatgaaacctcgcaaca-3′ Reverse 5′-ttcattgcagttgccgttgg-3′	197	NM_145947
Slc26a8	Forward 5′-ccaccggaaaccagctaatcc-3′ Reverse 5′-ttctcctcgttgtacacgctc-3′	123	NM_001290320
Slc26a9	Forward 5′-atcgcctcgctcatctttgc-3′ Reverse 5′-gggaaacccttgtcggatct-3′	204	NM_177243
Slc26a10	Forward 5′-ctgtggctggagtaaccgtg-3′ Reverse 5′-agaaagacgtgtagagtccga-3′	102	NM_177615
Slc26a11	Forward 5′-agtgtatgcgggaacacatgc-3′ Reverse 5′-cgaaggcgtaagcaatcagag-3′	141	NM_178743
Actb	Forward 5′-cactgtcgagtggcgtcca-3′ Reverse 5′-tgacccattcccaccatcac-3′	227	NM_007393.5
B2m	Forward 5′-taagcatgccagtatggccg-3′ Reverse 5′-ttgctatttctttctgcgtgc-3′	155	NM_009735
Kcnj13	Forward 5′-aatgctcctaggcctcatgc-3′ Reverse 5′-agacacgaacgttggtcaga-3′	191	NM_001110227