Fig. 3.

K⁺-deficient state with elevated muscle cell Na⁺ does not affect blood pressure or provoke inflammation. Mice were fed diet supplemented with 1% K⁺ (1K, ●) or diet without K⁺ (0K, ○) for 10 days. A: systolic blood pressure (SBP) was measured at baseline and day 5 and day 10 (n = 5/group). B: flow cytometric quantification of total leukocytes (CD45⁺), T cells (CD3⁺), and macrophages (F4/80⁺) in the thoracic aorta from both groups (n = 5/group). C: albumin:creatinine ratio was measured in both groups by ELISA (n = 4/group). D: flow cytometric quantification of total leukocytes (CD45⁺), T cells (CD3⁺), and macrophages (F4/80⁺) in the kidney from both groups (n = 5/group). E: splenic CD4⁺ T-cell production of IL-17A and CD8⁺ T cell production of IFNγ were quantified in both groups by ELISA (n = 4–5/group). Data are expressed as means ± SE. Comparisons were performed using GraphPad Prism parametric unpaired Student’s t test and P values are provided.