Fig. 1.

Body weight and composition of weight-cycling mice is comparable to mice not previously exposed to HFD. A: experimental design of weight cycling. B: body weight shows weight cycling causes reduction of body weight and then comparable regain of body weight to mice receiving HFD for first time. C: HFD→L→H mice have significantly lower epididymal fat mass but comparable total visceral fat and liver weights to LFD→H mice. DEXA was used to measure body fat in grams (g; D), body fat percentage (%; E), and lean mass (g; F) between groups. LFD, low-fat diet for 32 wk; HFD, high-fat diet for 32 wk; LFD→H, LFD for 28 wk and then changed to a HFD for 4 wk; HFD→L→H, HFD for 21 wk, then changed to LFD for 7 wk, and then changed to HFD for 4 wk. Data are shown as means ± SE (n = 8–10/group). Bar graphs not sharing a common letter are significantly different from one another according to one-way ANOVA (P < 0.05). Body weight measurements were tested against one another each week: %, HFD→L→H vs. LFD; #, HFD→L→H vs. HFD; $, HFD→L→H vs. LFD→H, &- HFD vs. LFD, @ - HFD vs. LFD→H; *, LFD→H vs. LFD indicate significance according to one-way ANOVA (P < 0.05).