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. Author manuscript; available in PMC: 2020 Nov 10.
Published in final edited form as: J Mol Endocrinol. 2020 Feb;64(2):53–65. doi: 10.1530/JME-19-0053

Figure 4.

Figure 4

Effect of the PPAR-α agonist, fenofibrate (FFB), D1-like receptor antagonist, LE300, and high NaCl concentration on gastrin expression in human stomach antrum. (A) FFB (5 μM/1 h) stimulates gastrin expression in human stomach antrum (P < 0.05, vs VEH, one-way ANOVA, n = 6, Tukey test). (B) 170 mM (4 h) sodium increases gastrin expression in intact human stomach G cells (P < 0.05, vs. VEH, one-way ANOVA, n = 4, Tukey test). The stimulatory effects of FFB and 170 mM NaCl tend to be blocked by the D1-like receptor antagonist LE300 but do not achieve statistical significance. (C) Immunostained human stomach antrum shows an increase in gastrin expression with fenofibrate (FFB, upper center image), relative to vehicle (VEH, upper left image). The D1R/D5R antagonist LE300 (10 μM/1 h) tends to block the stimulatory effect of FFB (upper right image). High extracellular NaCl (170 mM) (lower center image) also increases antrum gastrin staining, relative to VEH (lower left image). The D1R/D5R antagonist LE300 (10 μM/4 h) tends to block the stimulatory effect of 170 mM NaCl on gastrin expression (lower right image). (D) RT-PCR of gastrin in stomach antrum treated with FFB (5 μM/1 h) and 170 mM NaCl (4 h); LE300 blocks the stimulatory effect of 170 mM NaCl on gastrin mRNA but not the stimulatory effect of FFB (* and *** P < 0.05 vs VEH; #P < 0.05, NaCl + LE vs NaCl, one-way ANOVA, Tukey test). (E) Representative DNA gel image of gastrin and actin mRNA. All data are expressed as M ± s.e.