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. Author manuscript; available in PMC: 2021 Jan 1.

Published in final edited form as: Int J Hyperthermia. 2020;37(1):119–129. doi: 10.1080/02656736.2020.1714084

Figure 3: — A. In vitro heating of R3230 leads to increase in IL-6 secretion, demonstrated by ELISA. Bar charts indicate a significant increase in IL-6 secretion in medium of R3230 cells that were heated to 43°C for 5 min followed by incubation of 3hr or 8hr at 37°C. B. Western blot assay demonstrate an increase in phosphorylation of STAT3 following moderate hyperthermia (dense bands on gel electrophoresis at 80-kDa level as expected, after β-actin standardization). R3230 cells were heated to 43°C for 5 or 10min and incubated thereafter at 37°C for 3hr. Bar charts indicate a significant increase in STAT3 phosphorylation in cells that were heated for 5min. C. Western blot assay demonstrate increase in c-Met receptor protein following moderate hyperthermia seen as dense bands on gel electrophoresis at 50-kDa level, where c-Met receptor α subunit is expected, after β-actin standardization. Bar charts indicate a significant increase in c-Met receptor protein in R3230 cells that were heated both 5 and 10min at 43°C and incubated thereafter at 37°C for 8hr.

Figure 3: — A. In vitro heating of R3230 leads to increase in IL-6 secretion, demonstrated by ELISA. Bar charts indicate a significant increase in IL-6 secretion in medium of R3230 cells that were heated to 43°C for 5 min followed by incubation of 3hr or 8hr at 37°C. B. Western blot assay demonstrate an increase in phosphorylation of STAT3 following moderate hyperthermia (dense bands on gel electrophoresis at 80-kDa level as expected, after β-actin standardization). R3230 cells were heated to 43°C for 5 or 10min and incubated thereafter at 37°C for 3hr. Bar charts indicate a significant increase in STAT3 phosphorylation in cells that were heated for 5min. C. Western blot assay demonstrate increase in c-Met receptor protein following moderate hyperthermia seen as dense bands on gel electrophoresis at 50-kDa level, where c-Met receptor α subunit is expected, after β-actin standardization. Bar charts indicate a significant increase in c-Met receptor protein in R3230 cells that were heated both 5 and 10min at 43°C and incubated thereafter at 37°C for 8hr.