Fig. 4.
The MifS sensor domain is required for response to α-KG. Mutations were introduced into the sensor domain of MifS. The wild-type mifS gene or one of the mutated mifS genes were introduced into E. coli cells harbouring the mifR gene and the PPA5530::lacZ reporter. Cells were grown in LB media to an OD600 value of 0.3 and challenged with α-KG (black) or no substrate (white). LacZ expression levels were measured 60 min post-induction. Data points represent mean values±the standard deviations (n=3). Analysis of variance was performed by using Dunnett’s post-hoc test (α value of 0.05) to identify significant differences (P<0.0001; marked with an asterisk).