Table 1.
SARS-CoV-specific antisera used and their cross-reactivity with corresponding SARS-CoV-2 targets
|
SARS-CoV antiserum |
Function of target |
Antigen type |
Antibody type |
IFA signal* |
Reference |
|---|---|---|---|---|---|
|
nsp3 (DGD7) |
transmembrane replicase protein, containing PLpro |
bacterial expression product |
rabbit polyclonal |
++ |
[48] |
|
nsp4 (FGQ4) |
transmembrane replicase protein |
synthetic peptide |
rabbit polyclonal |
++ |
[109] |
|
nsp5 (DUE5) |
Mpro |
bacterial expression product |
rabbit polyclonal |
+ |
[48] |
|
nsp6 (GBZ7) |
transmembrane replicase protein |
synthetic peptide |
rabbit polyclonal |
− |
[109] |
|
nsp8 (DUK4) |
RNA polymerase co-factor |
bacterial expression product |
rabbit polyclonal |
++ |
[48] |
|
nsp8 (39-12) |
RNA polymerase co-factor |
bacterial expression product |
mouse monoclonal |
++ |
unpublished |
|
nsp9 (HLJ5) |
RNA-binding protein |
synthetic peptide |
rabbit polyclonal |
++ |
unpublished |
|
nsp13 (CQS2) |
RNA helicase |
synthetic peptide |
rabbit polyclonal |
++ |
[48] |
|
nsp15 (HLT5) |
endoribonuclease |
bacterial expression product |
rabbit polyclonal |
+ |
unpublished |
|
nsp15 (BGU6) |
endoribonuclease |
synthetic peptide |
rabbit polyclonal |
+ |
[48] |
|
M (EKU9) |
membrane protein |
synthetic peptide |
rabbit polyclonal |
+ |
[48] |
|
N (JUC3) |
nucleocapsid protein |
bacterial expression product |
rabbit polyclonal |
+ |
[35] |
|
N (46-4) |
nucleocapsid protein |
bacterial expression product |
mouse monoclonal |
++ |
[41] |
* ++, strongly positive; +, positive; -, negative.