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. 2020 Nov 10;15(11):e0242177. doi: 10.1371/journal.pone.0242177

Fig 1. Image quality and information enhancement from 2D to 3D.

Fig 1

(A) PD1+ (blue) and alpha-actin+ (red) cells in standard 2D specimen. The germinal centre is situated in the middle of the picture, surrounded by the T-zone. (B) Confocal microscope image shows follicular dendritic cells (FDC) in red (CD23+) and fibroblastic reticular cells (FRC) in green (alpha-actin+). (C) Constructed surfaces for the confocal microscopy image in Image 2 (FDC in red and FRC in green). This surface reconstruction illustrates the three-dimensionality of the tissue. As can be seen, the walls in B-/T-border are alpha-actin positive structures that can be related with the FRCs. Magnification of microscopy images. (A) 40x (B and C) 63x.