Table 4. Primer design, position of SNPs located in their respective gene and the protein sequence alteration.

SNP	Gene	Mutation	Chr	Pos	Protein	Primer-F	Tm-R	Primer-R	Tm-F
15	BG_GLEAN_10022339	C->T	2	12878537	V → I	TACTCCTCGGCGTCTAACCA	60.0	TGAGAAGCAGCCTCTCGTTG	60.0
26	BG_GLEAN_10022734	A->G	2	17634545	T → A	AGGTAGGCCCGAAGACTCAT	60.0	GGGAGGGCAGCTTGTTACTT	60.0
2	BG_GLEAN_10022642	C->A	2	16355258	S → Y	CGCGCTATGAAGGTCCAGAA	60.2	CCCAGGATTCTCAGCACACA	60.0
16	BG_GLEAN_10022589	A->G	2	15709303	K → E	GAGCCCAGTTGGTTCCTTGA	60.0	CATCTGCGATCTCCGTCCTG	60.0
31	BG_GLEAN_10022727	T->A	2	17457363	C → S	CGTCTATGGGGGTTGGCAAT	60.1	CGAGAGTCCTGCAGCAGAAA	60.0