Figure 2.

Relative expression of anti-oxidant genes in neuroblastoma cells treated with gracilin A derivatives. 150 μM H₂O₂ and compounds at μM concentrations were added to SH-SY5Y cells for 6 h and their effect on the expression of (a) catalase, (b) glutathione peroxidase 1, (c) superoxide dismutase 1, (d) superoxide dismutase 2 and (e) Nrf2 was evaluated. RPL0 was used as internal normalization control. Relative gene expression was calculated with ΔΔCt method. Cells treated only with H₂O₂ were used as calibrator and are represented by x-axes. Data are expressed as mean ±SEM of three independent replicates performed by duplicate and compared to H₂O₂ control cells by one way ANOVA and Dunnett’s tests. *p<0.05. **p<0.01, *** p<0.001