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. Author manuscript; available in PMC: 2020 Nov 10.
Published in final edited form as: ACS Chem Neurosci. 2019 Aug 23;10(9):4102–4111. doi: 10.1021/acschemneuro.9b00329

Figure 6.

Figure 6.

Gracilin A derivatives modulated the expression of Nrf2 and NFκB-p65 in microglia. BV2 murine cells were pre-treated with compounds at μM concentrations for 1 h. Then, LPS was added for 24 h in order to activate the cells. The expression levels of Nrf2 were measured in (a) the nucleus and (b) the cytosol. In the same way, NFκB-p65 expression was determined in (c) nuclear and (d) cytosolic fractions. Protein levels were normalized with lamin B1 and β-actin in the nucleus and the cytosol, respectively. Results are mean± SEM of three replicates performed by duplicate. Statistical differences were calculated with one way ANOVA and Dunnett’s test. Treatments with compounds are compared to LPS control cells (*p<0.05, **p<0.01, ***p<0.001) and LPS control cells are compared to inactivated cells (#p<0.05, ##p<0.01, ###p<0.001)