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. 2020 Oct 26;9:e60234. doi: 10.7554/eLife.60234

Figure 7. Shh-dependent cell fates expand laterally in Ift122 and Ttc21b mutants.

Figure 7.

(A) Nkx6.1 protein visualized in tiled confocal images of cranial neural plate cells labeled with phalloidin (F-actin). (B,C) Nkx6.1 intensity plotted by distance from the midline, normalized to the mean Nkx6.1 intensity of the image, in Ift122 (B) and Ttc21b (C) mutants compared with wild-type littermate controls (WT). A single value was obtained for each embryo and the mean ± SD between embryos is shown, n = 3 embryos/genotype. Anterior up. Bars, 100 μm.