(
A) Micro-C detects more cohesin-dependent chromosome contacts with a smaller size-range than Hi-C. Interactions-versus-distance decaying curve shows the normalized contact density (y-axis) against the distance between the pair of crosslinked chromosomal loci from 3 kb to 1 mb (x-axis) for wild-type arrested in mitosis (
WT in blue), and cohesin-inactivated cells arrested in mitosis (cohesin-inactivated in red) in our Micro-C XL, Hi-C in
Schalbetter et al., 2017, and Hi-C in
Dauban et al., 2020 (top). Plot of the variation of the slope in the interactions-versus-distance decaying curve for the same datasets (bottom). (
B) Comparison of the plots of the variation of the slope in the interactions-versus-distance decaying curve for our Micro-C XL in wild-type (WT in blue) and without cohesin (cohesin-inactivated in red), and Hi-C in
Dauban et al., 2020 in wild-type (WT in grey) and without cohesin (cohesin-inactivated in green). (
C) Differential slopes of contact decay curves between cohesin inactivation and wild-type. Differential slopes were obtained by the subtraction of the slopes of cohesin inactivation to wild-type (y-axis) across the range of 1 kb to 50 kb (x-axis), showing the data from Micro-C XL in purple,
Schalbetter et al., 2017 in green, and
Dauban et al., 2020 in yellow.