Figure 1.

Mammary gland tumor cell culture (UNESP-CM9). (A) Culture of mammary gland cancer cells at passage 5 (P5). Note the heterogeneity in cell morphology, with some cells showing spindle morphology (asterisk) and other cell groups showing polygonal morphology (arrows). (B) Cell culture after selective trypsinization with polygonal morphology (compatible with epithelial cells) and high cell density after 48 h of culture. (C) Culture of cells from cold trypsinization. Note that most of the cell population has a fusiform morphology (arrows), and there are few cells with polygonal morphology (arrowhead). Note the low cell density after 30 days of cell culture.