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. 2020 Oct 27;11:548604. doi: 10.3389/fimmu.2020.548604

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Copyright © 2020 Koudriavtseva, Stefanile, Fiorelli, Lapucci, Lorenzano, Zannino, Conti, D’Agosto, Pimpinelli, Di Domenico, Mandoj, Giannarelli, Donzelli, Blandino, Salvetti and Inglese

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Luminex Assay Principle. The sample is added to the mixture of colored beads coated with specific antibodies that bind the analyte of interest, the biotinylated detection antibodies in turn bind the analyte, and an analyte-antibody sandwich is formed. Streptoavidin conjugated with phycoerythrin (PE) binds biotinylated detection antibodies. The analysis of the beads is carried out either in a double laser flow with the Luminex 200 instrument or inside a magnet with the Luminex MAGPIX analyzer. The signal strength of phycoerythrin is directly proportional to the concentration of the specific analyte.