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. 2020 Oct 27;11:548604. doi: 10.3389/fimmu.2020.548604

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Copyright © 2020 Koudriavtseva, Stefanile, Fiorelli, Lapucci, Lorenzano, Zannino, Conti, D’Agosto, Pimpinelli, Di Domenico, Mandoj, Giannarelli, Donzelli, Blandino, Salvetti and Inglese

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Workflow of circulating miRNA profiling. For circulating miRNA profiling blood samples from patients will be processed. In particular, circulating RNA will be extracted from serum samples with a column-based extraction method. Total and small RNA quality will be assessed by Bioanalyzer. Then, total RNA will be labelled for the hybridization to Human miRNA Microarray Release 21 (Agilent) containing probes for 2549 human miRNAs. Microarray data will be subjected to bioinformatic analysis to identify a signature of miRNAs differentially expressed. Deregulated miRNAs pathway analysis and correlations analysis with clinical variables will be performed.