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OVX-induced bone loss enriches of Th17, decreasing Treg in lymphoid organ. The pool of (A, C) CD4+Foxp3+ Treg cells and (B, D) CD4+IL17+ Th17 cells in splenocytes and MLN were analyzed by FACS. (E, F) IL-17 and IL-10 in serum were quantitatively analyzed by ELISA. p values of less than 0.05 were considered statistically significant (* p <0.05, ** p <0.01, *** p <0.001). MLN: mesenteric lymph node. n = 12/group.
