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The activity and quantity of lysosomes were both downregulated by EGF treatment in FGCs. Lysosomal activity was detected by Lyso-Tracker red, bar=50 μm (A). The quantity of lysosomes was evaluated by immunostaining (B), GPR30 antibody (red), LAMP1 antibody (green), and DAPI (blue), shown in this panel, bar=10 μm. “Neg” indicates negative control (secondary antibodies only). Three independent replicates were performed for each experiment.
