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. 2020 Oct 27;12(20):20139–20151. doi: 10.18632/aging.103679

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Copyright: © 2020 Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Upregulation of circ_PVT1 induces migration and invasion of CC cells by regulating EMT. (A) The transfection efficiency was verified by RT-PCR. n=6, **P<0.01. (B) The CCK8 assay was performed in cervical cancer cell lines. n=8, *P < 0.05. (C) Clone formation assays in cervical cancer cell lines. (D) Wound healing assay was carried out for migration. (E, F) The migration and invasion were explored by transwell. n=6, *P<0.05. (G) The number of nodules was statistics for detecting lung metastasis. n=6, *P<0.05. (H) The expression of metastasis-associated protein, E-cadherin, Vimentin, N-cadherin and SNAIL were detected. n=6, *P<0.05. (I) Immunofluorescence representative images of E-cadherin, Vimentin, N-cadherin. n=6, *P<0.05, **P<0.01.