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. 2020 Nov 1;2020:8848930. doi: 10.1155/2020/8848930

Figure 4.

Figure 4

ox-LDL triggers oxidative stress in macrophages through Drp1-related mitochondrial fission. (a, b) Cellular ROS were measured in an immunofluorescence assay using a DCFHDA probe after ox-LDL and siRNA/Drp1 treatments. (c, d) Mitochondrial ROS were measured in an immunofluorescence assay using a MitoSOX probe after ox-LDL and siRNA/Drp1 treatments. (e–g) Activity of the antioxidative factors GSH, GPX, and SOD was measured through ELISA after ox-LDL and siRNA/Drp1 treatments. p < 0.05.