Fig. 2.

Effect of KRGE on ovarian apoptosis in DHEA-induced PCOS rats. (A-D) The level of apoptosis was measured using ovarian sections by TUNEL staining at day 20 after DHEA treatment. Sham (A), KRGE 150 (D), DHEA (B), and DHEA + KRGE 150 (C) groups. (E-F) Protein expression level of Bad, cleaved caspase-9, cleaved caspase-3 was measured in ovaries by immunoblot analysis at Day 20 after DHEA treatment (E) and quantified (F). Values represent the mean ± SEM. Scale bar = 100 μm. #p < 0.05 and ##p < 0.01 versus Sham group; ∗p < 0.05 and ∗∗p < 0.01 versus DHEA group. KRGE, Korean Red Ginseng extract; DHEA, dehydroepiandrosterone; PCOS, polycystic ovarian syndrome; SEM, standard error of mean; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.