Table 3.

Serum and hepatic parameters of oxidative stress in the Experiment 2, in which WT and Scly KO mice were fed a high-fat diet supplemented with selenite + selenomethionine for 9 weeks. Values are mean ± SEM. Two-way ANOVA was applied, and different letters represent statistical significance after Bonferroni post-hoc test. P-values under 0.05 were deemed significant; n = 4–6 per group. WT, wild type; KO, knockout; GPX, glutathione peroxidase; HNE, 4-Hydroxy-Trans-2-Nonenal.

MALES
	WT		Scly KO		2-way ANOVA
	0.25 ppm	0.5 ppm	0.25 ppm	0.5 ppm	Pinteraction	PSe	PGenotype
Serum
GPX activity (nmol/mL-1)	41.2±3.8a	43.6±1.8a,b	46.6±1.2b	45.8±2.4b	0.1666	0.4905	0.0027
Liver
GPX activity (nmol/mg-1)	13.3±0.2	13.3±0.1	13.4±0.08	13.4±0.3	0.908	0.7292	0.3062
HNE (mg/mL)	6.8±1.4a	7.6±3.4a	12.8±1.8b	7.9±2.1a	0.008	0.0495	0.0043
FEMALES
	WT		Scly KO		2-way ANOVA
	0.25 ppm	0.5 ppm	0.25 ppm	0.5 ppm	Pinteraction	PSe	PGenotype
Serum
GPX activity (nmol/mL-1)	40.4±0.8a	40.9±1.3a	42.2±1.1b	45.2±0.8c	0.0155	0.001	<0.001
Liver
GPX activity (nmol/mg-1)	13.3±0.3	13.3±0.2	13.4±0.08	13.5±0.3	0.6771	0.6771	0.4564
HNE (mg/mL)	10.4±2.2a	9.4±7.3a	20.4±4.3b	16.2±7.2b	0.293	0.524	0.0025