Table 4.
Hepatic gene expression of selenoprotein and metabolic enzymes of WT and Scly KO mice after feeding on a high-fat diet supplemented with selenite (Experiment 1), assessed by qPCR. Values are mean ± SEM, were normalized to Hprt1 mRNA levels, and presented as arbitrary units. Two-way ANOVA was used to compare averages. P-values under 0.05 were deemed significant; n = 4–6 per group.
| SELENITE | |||||||
|---|---|---|---|---|---|---|---|
| 0.25 ppm | 1 ppm | 2-way ANOVA (p-value) | |||||
| MALES | WT | Scly KO | WT | Scly KO | Pinteraction | PSe | PGenotype |
| Selenop | 413.8±42.9 | 382.1±24.6 | 390.3±33.1 | 388.8±61.2 | 0.7291 | 0.8478 | 0.705 |
| Gpx1 | 80.4±3.7 | 64.7±6.4 | 67.7±6.1 | 65.2±3.8 | 0.2289 | 0.2593 | 0.1024 |
| Txnrd1 | 1.8±0.6 | 1.6±0.05 | 2.1±0.7 | 1.6±0.7 | 0.5055 | 0.4433 | 0.1887 |
| Pparg | 0.1±0.02 | 0.4±0.1 | 0.2±0.05 | 0.5±0.1 | 0.7851 | 0.7558 | 0.0063 |
| Selenbp2 | 3.2±0.8 | 1.7±0.4 | 3.9±0.7 | 1.3±0.3 | 0.3871 | 0.8573 | 0.0023 |
| Nfs1 | 0.3±0.05 | 0.3±0.04 | 0.4±0.05 | 0.4±0.05 | 0.4112 | 0.083 | 0.9016 |
| Ccbl2 | 2.5±0.9 | 1.2±0.3 | 2.9±0.5 | 1.5±0.4 | 0.9311 | 0.2877 | 0.0001 |
| 0.25 ppm | 1 ppm | 2-way ANOVA (p-value) | |||||
| FEMALES | WT | Scly KO | WT | Scly KO | Pinteraction | PSe | PGenotype |
| Selenop | 874.0±186.8 | 517.7±104.4 | 757.2±355.9 | 584.2±55.7 | 0.3369 | 0.7895 | 0.0113 |
| Gpx1 | 66.7±4.8 | 55.6±13.1 | 105.0±50.5 | 67.4±13.0 | 0.2976 | 0.0582 | 0.0653 |
| Txnrd1 | 1.6±0.7 | 2.1±0.3 | 2.4±1.6 | 2.8±1.1 | 0.9452 | 0.1451 | 0.2847 |
| Pparg | 0.3±0.1 | 0.5±0,3 | 0.3±0.2 | 0.6±0.2 | 0.3533 | 0.4922 | 0.0083 |
| Selenbp2 | 2.6±1.4 | 3.7±1.0 | 2.5±1.6 | 3.6±1.2 | 0.9897 | 0.8237 | 0.0851 |
| Nfs1 | 0.3±0.06 | 0.5±0.05 | 0.4±0.08 | 0.5±0.05 | 0.2237 | 0.3922 | 0.03 |
| Ccbl2 | 2.2±0.6 | 4.0±1.1 | 4.9±2.5 | 3.6±0.8 | 0.0444 | 0.1007 | 0.7041 |