Table 5.
Hepatic gene expression of selenoprotein and metabolic enzymes of WT and Scly KO after feeding on a high-fat diet supplemented with a mixture of selenite + selenomethionine (Experiment 2) assessed by qPCR. Values are mean ± SEM, were normalized to Hprt1 mRNA levels, and presented as arbitrary units. Two-way ANOVA was used to compare averages. P-values under 0.05 were deemed significant; n = 4–6 per group.
| SELENITE + SEMET | |||||||
|---|---|---|---|---|---|---|---|
| 0.25 ppm | 0.5 ppm | 2-way ANOVA (p-value) | |||||
| MALES | WT | Scly KO | WT | Scly KO | Pinteraction | PSe | PGenotype |
| Selenop | 618.0±47.6 | 406.6±48.1 | 425.1±65.7 | 441.9±56.1 | 0.0552 | 0.1744 | 0.0978 |
| Gpx1 | 73.1±4.7 | 48.0±6.3 | 53.0±8.1 | 51.6±8.3 | 0.1066 | 0.2441 | 0.0749 |
| Txnrd1 | 4.3±1.4 | 4.7±1.5 | 4.5±2.5 | 5.5±2.6 | 0.6958 | 0.5343 | 0.4542 |
| Pparg | 0.2±0.04 | 0.4±0.05 | 0.2±0.09 | 0.4±0.08 | 0.7356 | 0.7312 | 0.0006 |
| Selenbp2 | 10.9±3.6 | 3.1±0.4 | 8.2±1.8 | 3.2±0.5 | 0.4553 | 0.4925 | 0.0028 |
| Nfs1 | 0.5±0.09 | 0.4±0.08 | 0.4±0.03 | 0.5±0.02 | 0.6673 | 0.571 | 0.9033 |
| Ccbl2 | 3.8±1.6 | 3±0.9 | 3.3±1.2 | 3.9±1.3 | 0.2109 | 0.7612 | 0.8552 |
| Cth | 6.0±0.7 | 5.7±0.5 | 5.3±0.3 | 5.0±0.5 | 0.9093 | 0.2389 | 0.5703 |
| 0.25 ppm | 0.5 ppm | 2-way ANOVA (p-value) | |||||
| FEMALES | WT | Scly KO | WT | Scly KO | Pinteraction | PSe | PGenotype |
| Selenop | 658.8±86.1 | 958.4±145.6 | 999.5±122.3 | 713.2±30.4 | 0.0132 | 0.6593 | 0.9508 |
| Gpx1 | 76.3±36.0 | 125.7±41.6 | 109.4±222.2 | 81.6±25.2 | 0.0116 | 0.6916 | 0.441 |
| Txnrd1 | 2.9±0.7 | 4.3±2.2 | 3.9±0.9 | 2.6±1 | 0.0294 | 0.5586 | 0.8339 |
| Pparg | 0.3±0.1 | 0.7±0.2 | 0.3±0.1 | 0.6±0.2 | 0.9564 | 0.5791 | 0.0021 |
| Selenbp2 | 2.7±0.8 | 4.1±0.5 | 2.8±+0.8 | 3.6±0.5 | 0.316 | 0.7191 | 0.0016 |
| Nfs1 | 0.5±0.2 | 0.8±0.08 | 0.6±0.1 | 0.7±0.08 | 0.0939 | 0.7087 | 0.0066 |
| Ccbl2 | 3.7±0.9 | 5.5±2.5 | 5.9±0.9 | 3.4±1.0 | 0.0037 | 0.9297 | 0.5748 |
| Cth | 5.9±1.5 | 11.1±2.3 | 6.5±0.8 | 9.0±2.7 | 0.1138 | 0.3548 | 0.0001 |