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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: J Biochem Mol Toxicol. 2020 Jun 24;34(11):e22561. doi: 10.1002/jbt.22561

FIGURE 2.

FIGURE 2

Time-dependent activation of the PERK, IRE1α, and ATF6 arms of the UPR by PFOA. A, Immunoblot analysis for protein expression of phosphorylated eIF2α, total eIF2α, Atf4, and Chop following treatment of 266-6 cells with DMSO (0) or 50 µg/mL PFOA for the indicated time points. Gapdh expression is shown for verification of equal protein loading. Shown is a representative experiment (n =4). Noted are the phosphorylated forms of Atf4 detected by the Atf4 antibody while NS indicates recognition of a non-specific band. B-D, qRT-PCR analysis was performed to determine mRNA expression of gene targets specific for the PERK, IRE1α, and ATF6 arms of the UPR pathways following treatment of 266-6 cells with DMSO or 50 µg/mL PFOA for the various time points indicated, or 1µM thapsigargin (TG) for 6 hrs. Shown is the average fold-induction ± std dev of measurements performed in triplicate from a representative experiment with DMSO values set to 1.0 (n = 3). * indicates significantly different from DMSO control p < 0.01; # indicates significantly different from DMSO control p < 0.05.