Figure 2.
PRS Locus EC1.45 and EC1.25 Are Active in hCNCCs and during Mouse Craniofacial Development
(A) ChIP-seq and ATAC-seq for PRS locus putative enhancer clusters EC1.45 (p300 Peak1 and Peak2), EC1.35 (S1 and S2), and EC1.25 (S3–S6).
(B) Luciferase reporter assays for EC1.45, EC1.35 (S1 and S2) and EC1.25 (S3–S6) in hCNCCs (left) and hESCs (right).
(C) Schematic outlining craniofacial domains at E9.5 and E11.5. BA1-2, branchial arch 1-2; FNP, frontonasal prominence; LNP, lateral nasal process; MdP, mandibular process; MNP, medial nasal process; MxP, maxillary process.
(D) In situ hybridization (ISH) for Sox9 at E9.5 and E11.5.
(E) Mouse LacZ reporter assay for EC1.45, EC1.35 (S1 and S2), and EC1.25 (S3–S6 tested individually) at E9.5 and E11.5.
(F) HREM for an EC1.45 LacZ reporter embryo at E11.5 (frontal view, top; parasagittal section, bottom). White arrow, activity in the MdP.