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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Exp Eye Res. 2020 Sep 7;200:108204. doi: 10.1016/j.exer.2020.108204

Figure 4: Spectacle investigation of bipolar cell subsets.

Figure 4:

A. Of 23,429 total cells, three clusters were identified as bipolar cells. B. Heatmaps were used to classify the three bipolar cell clusters as cone OFF bipolar cells (by expression of GRIK1), cone ON bipolar cells (by expression of GRM6), and rod bipolar cells (by expression of PRKCA), which are supported by violin plots (C). D. In order to identify potential subpopulations of bipolar cells, re-clustering of the bipolar cell populations was performed. E. RELN expression, which is enriched in the BC7 subclass of cone ON bipolar cells, did not localize to any subpopulation within the unprocessed data (insert) but localized to a subpopulation within the re-clustered bipolar cell object (dashed line). F. ERBB4 expression, which is enriched in the BC3A subclass of cone OFF bipolar cells, did not localize to any subpopulation within the unprocessed data (insert) but did localize to a subpopulation within the re-clustered bipolar cell object (dashed line).