Figure 2. Assembly of the V₁ and V_o Complex.

(A) Ribbon diagram of the V₁ complex with each subunit colored as in Figure 1.

(B) The A₃B₃ hexamer surrounded by SidK (grey). The A₃B₃ hexamer is composed of three pairs of AB heterodimer in three conformations, closed, semi-open (semi) and open.

(C) Ribbon diagram of the central stalk that is composed of subunit D (red) and F (cyan). The proline containing kink in subunit D is indicated by an arrow.

(D) Conformational differences of the three peripheral stalks, with the CTDs of subunit E (purple) and subunit G (pink) aligned.

(E) Comparison between human and rat V-ATPase structures at the region where H is localized.

(F) Ribbon diagram of the V_o complex with each subunit colored as in Figure 1.

(G) Ribbon diagram of c-ring (blue) with CTD of subunit a (wheat), e (green) and RNAseK (cyan). The c” subunit is colored in marine.

(H) Interactions of subunit d (green) with the DF stalk (red and cyan) and the c-ring (blue).

See also Figure S3.