KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Pen Strep	GIBCO	Cat# 15140-122
Freestyle 293 medium	GIBCO	Cat# 12338-018
Fetal bovine serum	GIBCO	Cat# 16000-044
Lauryl Maltose Neopentyl Glycol	Anatrace	Cat# NG310
Cholesteryl hemisuccinate	Anatrace	Cat# CH210
Critical Commercial Assays
Superdex 200, 10/300 GL	GE Healthcare	Cat# 17-5175-01
Deposited Data
V-ATPase (state 1) density map	This study	EMD-21847
V-ATPase (state 1) atomic model	This study	PDB 6WM2
V1 density map	This study	EMD-21845
V1 atomic model	This study	PDB 6WLZ
Vo density map	This study	EMD-21844
Vo atomic model	This study	PDB 6WLW
V-ATPase (state 2) density map	This study	EMD-21848
V-ATPase (state 2) atomic model	This study	PDB 6WM3
V-ATPase (state 3) density map	This study	EMD-21849
V-ATPase (state 3) atomic model	This study	PDB 6WM4
Experimental Models: Cell Lines
HEK293F GnTI-	ATCC	Cat# CRL-3022
Recombinant DNA
SidK (14-273)	pET28a	N/A
Software and Algorithms
SerialEM	(Mastronarde, 2005)	http://bio3d.colorado.edu/SerialEM
RELION	(Scheres, 2012)	https://www2.mrc-lmb.cam.ac.uk/relion
UCSF Chimera	(Pettersen et al., 2004)	http://www.cgl.ucsf.edu/chimera
PHENIX	(Adams et al., 2010)	https://www.phenix-online.org
COOT	(Emsley and Cowtan, 2004)	http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PyMOL	Molecular Graphics System, Version 1.8 Schrodinger	http://www.pymol.org
Maxquant	Maxquant 1.6.3.4	https://www.maxquant.org
Other
400 mesh Lacey carbon grids	Electron Microscopy Sciences	LC400-Cu-100