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. Author manuscript; available in PMC: 2021 Feb 3.
Published in final edited form as: Nat Biomed Eng. 2020 Aug 3;4(11):1030–1043. doi: 10.1038/s41551-020-0594-x

Fig. 2: Rescue and sequence analysis of antibody genes from ZIKV E antigen-specific human B cells.

Fig. 2:

a, Flow cytometric identification of target-specific memory B cells after labeling of magnetically enriched total B cells with ZIKV E antigen. Target-specific B cells were labeled with biotinylated recombinant E protein and detected by fluorochrome-conjugated streptavidin as indicated. Green arrow indicates FACS-sorted cells. Representative data of four experiments is shown. b, Bioinformatics filtering steps to select mAb sequences for synthesis. Timeline to identify sequences of target mAbs and obtain cDNA constructs for recombinant mAb expression is indicated with blue arrow in the timeline chart.