Fig 4.
HBV activates PI3K/AKT pathway by regulating m6A modification on PTEN mRNA. (A) qRT-PCR quantification of PTEN mRNA levels normalized to GAPDH in liver biopsies from HBV-negative patients (HCC-1 to HCC-3) or HBV-positive (HCC-4 and HCC) HCC patients and healthy controls (N-1 and PHH). (B) MeRIP-qRT-PCR analysis of total RNA from liver biopsy samples in (A) using primers specific to PTEN mRNA. (C) HBV expressing HepG2 cells (HepAD38, HBV off and on, and transfected with HBV 1.3-mer) were treated with LPS. After 16 h post-treatment, the cells were harvested to assess expression levels of PTEN mRNA and indicated proteins by qRT-PCR and immunoblotting. *P = 0.00065 ** P = 0.00010 *** P = 0.0017 **** P = 0.00078