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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Cell Immunol. 2020 Sep 30;358:104224. doi: 10.1016/j.cellimm.2020.104224

Figure 4. The HPi2 antigen is widely expressed in multiple tissues, including activated T cells, providing a constant tonic signaling.

Figure 4

(A) Microscopy of T cells transduced with HPi2-CAR or empty vector (Mock). (B) Growing curve of CD4+ and CD4+CD25+ T cells after transduction with HPi2-CAR or Mock plasmid. 1×106 isolated T cells were stimulated with anti-CD3 for 48 hrs days before the transduction (arrowhead). (C) Immunofluorescence of tissue array with anti-HPi2 (red); Blue – DAPI. MO – medulla oblongata; SKM – skeletal muscle; SMM – smooth muscle; AG – adrenal gland. (D) FACS analysis of CD4+CD25+HPi2+ cells within the isolated peripheral blood mononuclear cells (PBMCs)in live lymphocytes gate. HPi2 control – the secondary antibody only