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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Cell Immunol. 2020 Sep 16;358:104216. doi: 10.1016/j.cellimm.2020.104216

Figure 2. Transduction efficiency in murine CD4+ T cells by lentivirus and retrovirus pseudotyped with different envelop proteins.

Figure 2.

(A) Viral vectors including VSVG and ecotropic envelop-pseudotyped retroviral vectors and VSV-G and Cocal envelop-pseudotyped lentiviral vectors were tested to increase transduction in murine CD4+ T cells. (B) Fixable viability dye eFluor 450 was adopted to decrease anti-FVIII-FITC background produced by dead cells. Anti-FVIII and Foxp3 were gated on CD4+ viable T cells. F8CAR-LV and F8CAR-Foxp3-LV pseudotyped with Cocal envelop protein showed the best transduction efficiency in murine CD4+ T cells. In addition, CD25 were gated on transduced cells to measure T cell activation.