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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Fertil Steril. 2020 Aug 28;114(5):1085–1096. doi: 10.1016/j.fertnstert.2020.05.036

FIGURE 3.

FIGURE 3.

HMGA2 promotes angiogenesis and mediates IGF2BP2/pAKT activity in human umbilical vein endothelial cell (HUVEC) migration. (A) Tube formation of HUVEC in the CM from LM and MM cells without (control) and with HMGA2 overexpression. (B) Histogram demonstrating the number of complete tubes and significance analysis. (C) Histogram showing the number of migrated HUVEC (Transwell assay) in the CM from LM and MM cells without (control) and with HMGA2 overexpression. (D) Histogram showing the relative migration rate of HUVEC after scratching in the CM from LM and MM cells without (control) and with HMGA2 overexpression. *P<.05; **P<.01. (E) Western blot analysis illustrating pAKT and IGF2BP2 expression in LM and MM cells with and without HMGA2 overexpression. (F) Expression analysis of HMGA2 and pAKT in LM and MM cells after silencing IGF2BP2 expression. (G, H) HUVEC migration analysis in the CM from LM and MM cells without (control) and with HMGA2 overexpression, as well as silencing IGF2BP2.