Figure 1.

TLR2 levels in A549 cells incubated with P. brasiliensis yeasts or PAM₃CSK₄. (a,b) A549 cells were incubated or not (C) with P. brasiliensis yeasts (MOI = 2.5) (Pb). (c,d) A549 cells were incubated or not (C) with 5 μg/mL of the TLR2-specific ligand PAM₃CSK₄ (PAM). After 5 h or 24 h, epithelial cells were lysed and aliquots were submitted to SDS–PAGE. TLR2 expression was analysed by Western blot. β-actin was used as loading protein control. Relative TLR2 levels were determined by densitometric analysis of the bands. Values represent the ratio of the intensity of TLR2 band divided by the corresponding intensity of β-actin band. Similar results were obtained from three independent experiments. Dashed lines in (a,b) represent cropped images. Uncropped images are presented in Supplementary Fig. S1.