Figure 3.

Interaction of α3 integrin with TLR2 in A549 cells infected with P. brasiliensis yeasts. A549 cells were incubated (Pb) or not (C) with P. brasiliensis yeasts (MOI = 2.5). After 5 h (a) or 24 h (b), cells were lysed and samples were incubated with anti-α3 integrin (α3 int) antibodies (IP). After 16 h, agarose beads conjugated with A/G protein were added. Then, sample buffer was added, and the resultant supernatant was submitted to SDS–PAGE. TLR2 was analysed by Western blot (WB). Similar results were obtained from three independent experiments. Uncropped images are presented in Supplementary Fig. S3.