Figure 6.

α3 integrin levels in A549 cells infected with P. brasiliensis yeasts using a Transwell system. A549 cells were incubated with P. brasiliensis yeasts (MOI = 2.5), which were in direct contact with epithelial cells (Pb) or disposed in the upper compartment of a Transwell system (Pb_UP). Control (C) was performed in the absence of yeasts. After 24 h, epithelial cells were lysed and aliquots were submitted to SDS–PAGE. α3 integrin levels were analysed by Western blot. β-actin was used as loading protein control. Similar results were obtained from three independent experiments. Dashed lines represent cropped images. Uncropped images are presented in Supplementary Fig. S6.