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. 2020 Nov 10;6:119. doi: 10.1038/s41420-020-00356-1

Fig. 1. Butyrate-induced multiple types of regulated cell death in PDLFs.

Fig. 1

A PDLFs were pretreated with a pan-caspase inhibitor (10 μM Z-VAD-FMK), a RIP1 inhibitor (20 μM necrostatin-1), an MLKL inhibitor (10 μM necrosulfonamide) or lipid peroxide scavengers (5 μM ferrostatin-1) for 2 h, and then cells were stimulated with butyrate (8 mM) for 48 h. B PDLFs were incubated with 1, 5, or 10 μM ferrostatin-1 and 50, 100 μM DFO for 2 h, and then treated with 20 μM RSL3 for 12 h or 20 μM Erastin for 24 h before pretreatment with 1, 5, or 10 μM ferrostatin-1 and 50, 100 μM DFO for 2 h. Cell death was assessed by LDH assay. Mean ± SD, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control group.