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Primers used in this study
| Primers | Sequences (5′–3′)a |
|---|---|
| BTL2-F | GCCATGGATGGCAAGTCCGCGCGCAA (Nco I) |
| BTL2-R | GGCTCGAGTTAATGGTGATGGTGATGATGCGGACG (Xho I) |
| MCS-F | CGAAATTAATACGACTCACTATAGG |
| MCS-R | CCCAAGGGGTTATGCTAGTTATTGC |
aRestriction enzymes site were marked in italic
